Abstract
The yeast strain Metschnikowia persimmonesis Kang and Choi et al., sp. nov. [type strain KIOM_G15050 = Korean Collection for Type Cultures (KCTC) 12991BP] was isolated from the stalk of native persimmon cultivars (Diospyros kaki Thumb) obtained from different regions of South Korea and was characterized phenotypically, genetically, and physiologically. The isolate grew between 4 and 40 °C (optimum temperature: 24–28 °C), pH 3–8 (pH optimum = 6.0), and in 0–4% NaCl solution (with optimal growth in absence of NaCl). It also exhibited strong antibiotic and antimicrobial activities. Morphologically, cells were characterized by the presence of long, needle-shaped ascospores. Based on 18S ribosomal DNA gene sequence analysis, the new species was found to belong to the genus Metschnikowia as a sister clade of Metschnikowia fructicola. We therefore conclude that this yeast isolate from D. kaki is a new member of the genus Metschnikowia and propose the name M. persimmonesis sp. nov. This strain has been deposited in the KCTC for future reference. This discovery provides a basis for future research on M. persimmonesis sp. nov., including its possible contribution to the medicinal properties of the host persimmon plant.
Highlights
The genus Metschnikowia of the family Metschnikowiaceae (Order Saccharromycetales) comprises single-celled species that reproduce via budding of vegetative cells and is characterized by the presence of one or two needle-shaped ascospores on elongated asci (Mendonca-Hagler et al 1993; Pretorius 2000; Marinoni et al 2006; Kuan et al 2016)
We describe a new yeast species Metschnikowia persimmonesis Kang and Choi et al, sp. nov. isolated from the stalk of native Korean D. kaki cultivars obtained from different regions of South Korea
Growth curves characteristics of M. persimmonesis in potato dextrose broth (PDB) and LB medium Metschnikowia persimmonesis that grew in potato dextrose agar (PDA) and tryptic soy agar (TSA) media (Fig. 3) was used for growth curve generation and strain characterization
Summary
The genus Metschnikowia of the family Metschnikowiaceae (Order Saccharromycetales) comprises single-celled species that reproduce via budding of vegetative cells and is characterized by the presence of one or two needle-shaped ascospores on elongated asci (Mendonca-Hagler et al 1993; Pretorius 2000; Marinoni et al 2006; Kuan et al 2016). M. persimmonesis was identified as a novel species based on pyrosequencing of its 18S rDNA gene. We characterized this species morphologically, genetically, and physiologically, and evaluated its growth in the presence of various nutrients and environmental stimuli. Its taxonomic membership was confirmed by a phylogenetic analysis that revealed M. fructiola as the closest sister species. This novel yeast strain has been given Korean Patent Submission Number (10-2016-0137873) and international Patent Submission Number (PCT-KR2017-010681) and deposited in the Korean Collection for Type Cultures (KCTC) for future reference and research
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