Abstract
Botryosphaeria dothidea is, globally, one of the most economically important phytopathogenic fungi worldwide, causing the canker and dieback of fruit trees. An increasing number of viruses infecting B. dothidea have lately been reported, several of which could confer hypovirulence. In this study, isolated from strain ZM170285-1 of B. dothidea, a novel double-stranded RNA (dsRNA) mycovirus, tentatively named Botryosphaeria dothidea partitivirus 2 (BdPV2), was identified well. The BdPV2 harbored three dsRNA segments (1–3) with lengths of 1751, 1568, and 1198 bp, which encoded an RNA-dependent RNA polymerase (RdRp), a capsid protein (CP), and a hypothetical protein of unknown function, respectively. BLASTp searches revealed that the predicted protein sequences of dsRNA1 and dsRNA2 had the highest identities (74.95% and 61.01%) with the corresponding dsRNAs of Penicillium stoloniferum virus S (PsV-S), whereas dsRNA3 shared the highest identity (32.95%) with the dsRNA3 of Aspergillus ochraceous virus 1 (AoV1). Phylogenetic analysis indicated that BdPV2 belonged to the Gammapartitivirus genus and Partitiviridae family. To our knowledge, this is the first report of a Gammapartitivirus in B. dothidea.
Highlights
Mycoviruses are widely distributed and reported in most major groups of fungi [1,2,3]
BLASTp searched of P1 revealed that it shares the highest identity (74.95%, E-value 0, coverage 100%) with the RNA-dependent RNA polymerase (RdRp) of Penicillium stoloniferum virus S (PsV-S) and Penicillium brevicompactum partitivirus 1 (PbPV1)
BLASTp analysis revealed that P2 shared the highest identity (61.01%, E-value 0, coverage 100%) with the capsid protein (CP) of PsV-S and high identities (52.41–60.00%) with the CPs of the other members of the Partitiviridae family (Table 1)
Summary
Mycoviruses are widely distributed and reported in most major groups of fungi [1,2,3]. Some mycoviruses can cause the hypovirulence of hosts and, be used as biocontrol agents for certain fungal diseases [4,5,6]. Mycoviruses with dsRNA genomes were classified into seven families: Chrysoviridae, Partitiviridae, Reoviridae, Megabirnaviridae, Totiviridae, Quadriviridae, and Endornaviridae [11]. Members of the Partitiviridae family generally have two or three genome segments (1.4 to 2.4 kbp in length) encoding an RNA-dependent RNA polymerase (RdRp), a capsid protein (CP), and a hypothetical protein with unknown function, respectively [12]. Even if most partitiviruses exhibited no obvious effects on their host fungi [12], a few exceptions could interfere with the natural physiology, including morphology, toxin production, and hypovirulence [15,16,17]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
