Characterization of a Novel MicroRNA from the T-DNA Locus of Rosewood, an Activation-Tagged Poplar Tree

Abstract

Plant phenomics refers to using the phenotype of mutants to identify genes responsible for a trait of interest. Our lab has created a large population of mutant poplar trees for studying phenomics. Poplar plays an enormous role in biotechnology and biofuel production. By having a large population of organisms with various mutations, genes and interacting partners may be identified. Specifically, we can identify genes responsible for tree stature, wood development, growth rate and pest resistance. The mutant rosewood is characterized by a vibrant red wood due to an accumulation of pelargonidin, the chemical that gives strawberries and cherries their red colour. Previous work on the mutant has shed light on the possibility of a microRNA regulating the biosynthesis of pelargonidin. Elucidating the function of the potential microRNA and its downstream targets will provide insight for applications in plant biotechnology. As well, it will be the first experimental validation of a microRNA in plants that regulates wood properties.