Abstract
Background: Use of novel microorganisms for beneficial purposes is still remaining a challenging job. This study was designed to isolate, characterize and use of a novel hot spring bacterial strain from a virgin hot spring of Metaldanga, Birbhum, West Bengal, India. Methods: A pure bacterial strain (MDH1) was identified by growing the enrichment culture isolated from Metaldanga hot spring through serial dilution process in a semi-synthetic medium at pH 8.0 and 42°C temperature. The novelty of the strain was characterized by 16S-rRNA gene sequence analysis. The bacterium acted as template to synthesize spherical gold nanoparticles (GNPs). GNPs were characterized by using UV-Vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS) and Fourier transform infra-red spectroscopy (FTIR). Results: The phylogenetic analysis suggested that MDH1 strain (GenBank accession number: KT600031) was affiliated to the family “Pseudomonadaceae” with 99% homologous to Pseudomonas putida H8234. The coccoid shaped bacterium was gram-negative and facultative-anaerobic which acted as a template to synthesize spherical GNPs with an average size of 12 ± 3 nm when examined under transmission electron microscopy (TEM). FT-IR studies revealed the presence of bioactive functional groups which acted as capping and stabilizing agents of the GNPs. XRD pattern confirmed the amorphous nature of GNPs. The Zeta potential (ζ) concluded the adequate stability of GNPs in an aqueous environment. Conclusions: The present investigation explores the microbial diversity of a virgin hot spring of Metaldanga for its beneficial applications in industry, particularly in the synthesis of the gold nanoparticles.
Highlights
A hot spring or a hydrothermal spring comprises warm or hot groundwater which is heated by geothermal heat, the essential heat from earth’s interior
One of these strain which act as template for synthesizing gold nanoparticles was isolated and purified by serial dilution technique
The bacterial cell morphology was determined by Field Emission Scanning Electron Microscope (FESEM) which showed that the isolated bacterium was coccoid in shape (Figure 2)
Summary
A hot spring or a hydrothermal spring comprises warm or hot groundwater which is heated by geothermal heat, the essential heat from earth’s interior. The hot spring water contains dissolved solids and several elements such as calcium, lithium, radium, and sulphur Such geothermal hot spring streams provide a favourable condition for the development of microbial mats, which covers physiologically and phylogenetically different groups of prokaryotes, e.g., chemotrophic sulphur bacteria, cyanobacteria and oxygenic phototrophic bacteria, depending on the temperature, pH, sulphide concentration and some other environmental conditions [1]. The microorganisms belonging to the thermophilic microbial community can fulfil both degenerative and productive functions Such microorganisms probably carry out global reduction of metals and seem to play a major role in the deposition of minerals in the earth’s crust. This study was designed to isolate, characterize and use of a novel hot spring bacterial strain from a virgin hot spring of Metaldanga, Birbhum, West Bengal, India. Conclusions: The present investigation explores the microbial diversity of a virgin hot spring of Metaldanga for its beneficial applications in industry, in the synthesis of the gold nanoparticles
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