Abstract
A keratinophilic fungus Chrysosporium tropicum was isolated from poultry farm soil and screened for extracellular keratinase activity. The fungus was cultured in basal salts medium and keratinase production was assessed. The novel keratinase was purified by Sephadex G-100 column chromatography and characterized. The molecular weight of the enzyme was estimated to be 14.5 KDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimum pH and temperature of the keratinase were found to be 7.5 and 40oC respectively. The Km for keratin powder from human hair was 6.67 mg and the Vmax of novel keratinase was determined to be 0.33 mg ml-1 by the Lineweaver-Burk plot. The enzyme activity was almost completely inhibited by phenylmethylsulphonyl fluoride (PMSF) suggesting that the keratinase belongs to the serine protease family.
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