Characterization of a new ViI-like Erwinia amylovora bacteriophage phiEa2809.

Alexander L Lagonenko,Leonid N Valentovich,Olga Sadovskaya,Anatoly N Evtushenkov

doi:10.1093/femsle/fnv031

Alexander L Lagonenko, Leonid N Valentovich + Show 2 more

Open Access

https://doi.org/10.1093/femsle/fnv031

Copy DOI

Journal: FEMS Microbiology Letters	Publication Date: Feb 24, 2015
Citations: 19	License type: cc-by

Affiliation: Belarusian State University

Abstract

Erwinia amylovora is a Gram-negative plant pathogenic bacteria causing fire blight disease in many Rosaceae species. A novel E. amylovora bacteriophage, phiEa2809, was isolated from symptomless apple leaf sample collected in Belarus. This phage was also able to infect Pantoea agglomerans strains. The genome of phiEa2809 is a double-stranded linear DNA 162,160 bp in length, including 145 ORFs and one tRNA gene. The phiEa2809 genomic sequence is similar to the genomes of the Serratia plymutica phage MAM1, Shigella phage AG-3, Dickeya phage vB DsoM LIMEstone1 and Salmonella phage ViI and lacks similarity to described E. amylovora phage genomes. Based on virion morphology (an icosahedral head, long contractile tail) and genome structure, phiEa2809 was classified as a member of Myoviridae, ViI-like bacteriophages group. PhiEa2809 is the firstly characterized ViI-like bacteriophage able to lyse E. amylovora.

Highlights

Erwinia amylovora is a Gram-negative plant pathogenic bacteria causing fire blight disease in many Rosaceae species.A novel E. amylovora bacteriophage, phiEa2809, was isolated from symptomless apple leaf sample collected in Belarus
Fire blight, which is caused by plant pathogenic bacterium Erwinia amylovora, is a very destructive and economically significant disease of apples, pears and many other Rosaceae plants
Some of the described bacteriophages were highly specific towards E. amylovora, while others could infect related epiphytic and pathogenic bacteria, such as E. pyrifoliae and Pantoea agglomerans

Summary

Introduction

Fire blight, which is caused by plant pathogenic bacterium Erwinia amylovora, is a very destructive and economically significant disease of apples, pears and many other Rosaceae plants. Erwinia amylovora is a Gram-negative plant pathogenic bacteria causing fire blight disease in many Rosaceae species.A novel E. amylovora bacteriophage, phiEa2809, was isolated from symptomless apple leaf sample collected in Belarus. The genome of phiEa2809 is a double-stranded linear DNA 162 160 bp in length, including 145 ORFs and one tRNA gene.

Results

Conclusion