Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil

Sheila Conceição Maciel,José Segundo Giampan,Elliot Watanabe Kitajima,Jorge Alberto Marques Rezende,Ricardo Ferreira Da Silva,Marcelo Silva Reis,Luis Eduardo Camargo,Adriana Salomão Jadão,Daniel Dias Rosa

doi:10.1590/s0103-90162011000600013

Sheila Conceição Maciel, José Segundo Giampan + Show 7 more

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https://doi.org/10.1590/s0103-90162011000600013

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Journal: Scientia Agricola	Publication Date: Dec 1, 2011
Citations: 26	License type: cc-by

Affiliation: Universidad San Pedro

Abstract

Catharanthus roseus is a perennial, evergreen herb in the family Apocynaceae, which is used as ornamental and for popular medicine to treat a wide assortment of human diseases. This paper describes a new potyvirus found causing mosaic symptom, foliar malformation and flower variegation in C. roseus. Of 28 test-plants inoculated mechanically with this potyvirus, only C. roseus and Nicotiana benthamiana developed systemic mosaic, whereas Chenopodium amaranticolor and C. quinoa exhibited chlorotic local lesions. The virus was transmitted by Aphis gossypii and Myzus nicotianae. When the nucleotide sequence of the CP gene (768nt) was compared with other members of the Potyviridae family, the highest identities varied from 67 to 76 %. For the 3' UTR (286nt), identities varied from 16.8 to 28.6 %. The name Catharanthus mosaic virus (CatMV) is proposed for this new potyvirus.

Highlights

Catharanthus roseus is known as the common periwinkle or Madagascar periwinkle
The plant has historically been used in popular medicine to treat a wide assortment of human diseases, such as diabetes, high blood pressure, as it contains more than 150 useful alkaloids
Espinha and Gaspar (1997), for instance reported a mosaic disease of C. roseus caused by Cucumber mosaic virus (CMV), whereas Seabra et al (1999) described the occurrence of an uncharacterized potyvirus causing mosaic and leaf malformation in this species in São Paulo State (SP), Brazil

Summary

Introduction

Catharanthus roseus is known as the common periwinkle or Madagascar periwinkle. It is a perennial, evergreen herb in the family Apocynaceae, which grows up to 80 cm high; has glossy, dark green leaves and bloom during summer. The occurrence of plants of C. roseus exhibiting mosaic symptoms and leaf malformation associated to virus infection is very common in Brazil.

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Conclusion