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Abstract
Peripheral T cell lymphomas (PTCLs) are associated with a poor prognosis due to often advanced disease at the time of diagnosis and due to a lack of efficient therapeutic options. Therefore, appropriate animal models of PTCL are vital to improve clinical management of this disease. Here, we describe a monoclonal CD8+ CD4− αβ T cell receptor Vβ2+ CD28+ T cell lymphoma line, termed T8-28. T8-28 cells were isolated from an un-manipulated adult BALB/c mouse housed under standard pathogen-free conditions. T8-28 cells induced terminal malignancy upon adoptive transfer into syngeneic BALB/c mice. Despite intracellular expression of the cytotoxic T cell differentiation marker granzyme B, T8-28 cells appeared to be defective with respect to cytotoxic activity as read-out in vitro. Among the protocols tested, only addition of interleukin 2 in vitro could partially compensate for the in vivo micro-milieu in promoting growth of the T8-28 lymphoma cells.
Highlights
To develop novel therapeutic strategies for peripheral T cell lymphoma (PTCL) appropriate animal models are crucial [1]
Isolation and characterization of the T8-28 cell line from an un-manipulated BALB/c mouse will be instrumental in furthering our understanding of lymphomagenesis in mice and will help to develop successful therapies for PTCL in humans
T8-28 cells were initially isolated from an un-manipulated adult male BALB/c.OlaHsd mouse kept under standard housing conditions
Summary
To develop novel therapeutic strategies for peripheral T cell lymphoma (PTCL) appropriate animal models are crucial [1]. The splenocytes we had transplanted consisted by more than 95% of CD8ab+ CD42 CD3+ TCR+ and CD28+ T cells as revealed by fluorescence-activated cell sorting analysis (Figure 1B, D), indicating that we had isolated a T cell lymphoma which we termed T8-28.
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