Characterization of a murine thymic CD4+ T cell subset-TCRαβ+ 3G11− 6C10− CD4+ CD8− thymocytes

Abstract

The presence of a relatively mature CD4(+) CD8(-) (SP) T cell subset in mouse thymus has been demonstrated. Composing of 10% of total CD4SP thymocytes, this subset is defined by the absence of 3G11 and 6C10 expression with a phenotype of CD69(+/-), HSA(med/lo) and heterogeneous for Qa-2 expression. The proliferation capability of TCRalphabeta(+) 3Gl l(-) 6C10(-) CD4(+) CD8(-) thymocytes was high while using Con A stimulus. And Con A stimulation could result in secretion of IL4, IL-10, IL-6 and a little amount of IFNgamma. IL-2 was barely detectable. This is distinct from typical Th0 type cytokines. The cells of this subset were NK1.1 negative, but strongly expressed GATA-3 mRNA. The results suggest that the CD4(+) subset of 3G11(-) 6C10(-) NK1.1(-) phenotype possesses immunocompetent cells with functions characteristic of Th2-like cytokines, which may indicate the cells at transitional status from Th0 to Th2, with a propensity to Th2.