Characterization of a Molybdenum-reducing and Phenol-degrading Pseudomonas sp. strain Neni-4 from soils in West Sumatera, Indonesia

Abstract

Millions of tonnes of these chemicals are manufactured each year and a large quantity is determined to be contaminating the environment, making them important worldwide pollutants. The fact that they pollute the environment is a major problem on a worldwide scale. There is a continuing search for bioremediation of these contaminants employing bacteria capable of numerous detoxifications. Analysis of the bacterium yielded a preliminary identification of the organism as Pseudomonas aeruginosa Neni-4. Screening for the capacity of molybdenum-reducing bacteria to decolorize different polyphenols was conducted in this study. Reduction was optimum at pH 6.3 and between 25 and 40 oC. The bacterium used glucose as the best carbon source or molybdenum reduction followed by galactose, 2-ketogluconate, and citrate in decreasing order. Phosphate between 5.0 and 7.5 mM and sodium molybdate between 15 and 20 mM maximally supported reduction. Like earlier Mo-reducing bacteria, a reduction of phosphomolybdate is seen in the absorption spectra of the Mo-blue generated. Heavy metals prevented molybdenum reduction. None of the phenolic compounds can reduce molybdenum when provided as sole carbon sources. In contrast, the bacterium was able to grow on phenol, benzoate, salicylic acid, and catechol, all of which are substances that include phenolic components. A significant bioremediation technology is this bacterium's capacity to metabolise molybdenum and thrive on poisonous phenolics.