Abstract
We have begun to characterize the role of the lysophospholipid acyltransferase, 1881, in Candida albicans. 1881 is homologous to the LPT1 gene in S. cerevisiae. A cDNA copy of the 1881 gene has been generated and the four CUG codons changed to UCG so to allow maintenance of the primary structure upon expression and translation in a lpt1 S. cerevisiae strain. In parallel, a homozygous, C. albicans strain has been generated which has both 1881 alleles removed by homologous recombination. This strategy of over and abrogated expression will allow us to address the question, what is the substrate specificity of 1881 with respect to degree of unsaturation of acyl‐CoA species? We will also address the questions, does removal of 1881 from the genome affect C. albicans a) virulence in C. elegans or CaCo2 cell based assays? b) membrane composition? c) hyphal growth? d) sensitivity to azoles and other antifungals? and e) viability in hypoxia?
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