Abstract
L. monocytogenes is the third most common cause of bacterial meningitis in adults. Further understanding of the pathophysiology of listeria meningitis is needed to improve the prognosis.
Highlights
Listeria monocytogenes is a common cause of bacterial meningitis
Non-treatment model In the survival study, mice inoculated with 105, 106 and 107 colony forming units per milliliter (CFU)/ml L. monocytogenes sequence type 1 (ST1) showed minimal clinical signs of disease limited to diminished grooming and/ or a slightly hunched back. They had to be sacrificed based on weight loss, ≥ 25% according to the predefined endpoint in the clinical scoring (Fig. 1a), and considered not representable for a listerial meningitis model
Mice inoculated with 108 CFU/ml L. monocytogenes ST1 showed signs of illness 12 h after inoculation, consisting of discharge in eyes, a slightly hunched back, mildly diminished activity and/or piloerection, and had a median survival time of 46 h (IQR 34–52 h; Fig. 1b)
Summary
Listeria monocytogenes is a common cause of bacterial meningitis. Several mouse and rat listeria models have been developed to study invasive L. monocytogenes diseases including cerebral and meningeal infection, using oral [15,16,17], intravenous [18], intracerebral [19,20,21,22,23], or intracisternal inoculation methods [24,25,26]. Problems with reproducibility, limited disease progression, or iatrogenic structural damage, combined with a need for a single model in which most pathological features seen in human listerial meningitis can be measured, have created the need for development of a new animal model. We developed a listerial meningitis mouse model to counter these problems and compared infection with listerial ST1 with ST6
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
