Abstract
Hemipteran insects are the most devastating pest for different crops of high economic value. Colocasia esculenta tuber agglutinin (CEA), a mannose binding monocot lectin from araceae family was previously reported by the present group to be effective against some members of this class of pests. In the present study, efficacy of this potent lectin has been extended to cotton aphid (Aphis gossypii) which is becoming a highly damaging pest of cotton in recent days. Because, like other aphids, A. gossypii not only extracts the phloem fluid but also transmit disease causing viruses and add to the high degree of yield loss. Efficacy of the lectin on cotton aphid as well as other hemipteran insects prompted us further to clone the protein coding gene. Very little sequence information of this gene was available in the database. Hence, attempt had been made to study the protein through liquid chromatography-tandem mass spectrometry (LC-MS/MS) to have the detailed peptide information. On the basis of the peptide homology information obtained from LC-MS/MS the complete coding sequence of CEA was determined. The coding sequence corresponding to CEA was cloned further using primers designed on the basis of above information and genome walk technology for its potential utilisation in insect management programme.
Highlights
Hemipteran insects are one of the most damaging pests affecting a wide array of economically important plants
Colocasia esculenta tuber agglutinin (CEA), a mannose binding monocot lectin from araceae family was previously reported by the present group to be effective against some members of this class of pests
Earlier reports indicated about the efficacy of different plant lectins, namely, Galanthus nivalis agglutinin (GNA), Allium sativum leaf agglutinin (ASAL), Arum maculatum tuber lectin (ATL), Amorphophallus paeonifolius tuber agglutinin (AMTL) against
Summary
Hemipteran insects are one of the most damaging pests affecting a wide array of economically important plants. Plant lectins are a class of non-immugenic carbohydrate binding proteins often found to be effective against this group of insect pests. Earlier reports indicated about the efficacy of different plant lectins, namely, Galanthus nivalis agglutinin (GNA), Allium sativum leaf agglutinin (ASAL), Arum maculatum tuber lectin (ATL), Amorphophallus paeonifolius tuber agglutinin (AMTL) against various insect pests [4,5,6,7,8,9]. The mode of action of one such mannose-binding lectin Amorphophallus paeonifolius tuber agglutinin (AMTL) on sucking type of hemipteran pest described in detail by Mondal et al [9] with additional information on interaction of lectin with insect midgut receptor and predicted the cause of toxicity either through nutrient leaching or causing anti-absorption detrimental effect due to lectin binding
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
