Characterization of a gene encoding a manganese peroxidase from Phanerochaete chrysosporium

Abstract

The complete nucleotide (nt) sequence of a gene ( mnp-1) encoding manganese peroxidase isozyme 1 (MnP-1)(pI= 4.9) from Phanerochaete chrysosporium has been determined. The sequence of 2539 bp includes 526 bp of 5′-flanking sequence and 368 bp 3′ to the poly(A) site. Comparison of cDNA and genomic sequences indicates six introns varying in size from 57–72 bp. Intron splice-junction sequences all adhere to the GT---AG rule. The positions of the introns show little similarity to the intron positions in the closely related lignin peroxidase-encoding genes. The 5′ upstream region of the mnp-1 gene contains a TATAA elements and three inverted CCAAT elements (ATTGG) at nt positions −81, −181, −195, and −304, respectively, relative to the start codon. In addition, the mnp-1 gene contains three putative heat-shock (HS) elements similar to the consensus C--GAA--TTC--G sequence, and two consensus metal response elements located within 500 bp upstream from the start codon. Furthermore, Northern-blot analysis demonstrates that mnp gene transcription is regulated by HS.