Abstract
Cells of a pure strain of a denitrifying bacterium have been immobilized in κ-carrageenan. The influence of pH and temperature on the immobilized cells was determined, as well as the operational stability in a continuous gas-lift loop reactor. Several steady states at different nitrate loadings were reached with respect to activity and dry cell weight. The results show that the immobilized cells form a stable system which rapidly reacts to changes in substrate (nitrate) supply. The maximum conversion rate of the immobilized cells is higher than the rate usually observed with immobilized denitrifying cells but lower than in fluidized-bed systems with attached biomass. It is shown that with increasing immobilized concentration the K m value apparently increases to such an extent that for growing cells no zero-order kinetics may be assumed.
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