Abstract
A substantial amount of vitamin B12 (357 ± 42.7 μg/100 g) was detected in pacific bluefin tuna (Thunnus orientalis) liver using a microbiological assay method. To evaluate whether tuna liver contains true vitamin B12 or an inactive corrinoid compound, a corrinoid compound was purified and characterized. The purified compound was identical to that of authentic vitamin B12, judging by TLC and LC/ESI-MS/MS chromatograms, and 1H NMR spectral data. These results indicate that pacific bluefin tuna liver contains substantial amounts of true vitamin B12 and would be an excellent source of the vitamin.
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