Abstract
An L3T4(CD4)+ CTL clone specific for Friend virus-induced tumor FBL-3 was isolated, characterized and compared with a conventional Lyt-2(CD8)+ CTL clone. This clone L3.1 was obtained from the limiting dilution culture of splenic MLTC cells from a CB6F 1 mouse whose CD8+ T cells had been suppressed by an in vivo injection of anti-Lyt-2.2 njAb. The phenotype of clone L3.1 was sIg -, Thy-1.2 +, L3T4(CD4) +, Lyt-2 (CD8) -, and Ia - as determined by flow-cytometry. Northern blot analysis also confirmed that mRNA for L3T4(CD4), but not for Lyt-2 (CD8) was present in the total RNA of L3.1. The FBL-3specific killing activity of L3.1 was inhibited by anti-H-2D b mAb, and the tumor cells did not express class II MHC antigen, indicating that the recognition of tumor antigen by this CD4 + CTL clone was restricted by the class I MHC molecule on the tumor cells. Furthermore, the finding that anti-L3T4(CD4) mAb GK1.5 inhibited the specific and lectin-dependent nonspecific cytotoxicity of L3.1 suggested that CD4 molecules on this CTL clone are not ligand (MHC class II)-binding proteins, but are involved in signal transduction.
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