Characterization of a CC1153 PVL-producing community-acquired methicillin-resistant Staphylococcus aureus from a dog bite wound.

Edet Ekpenyong Udo,Tsonyu Ivanov Dimitrov,Bobby Noronha,Samar Saed Boswihi,Tina Verghese,Bindu Mathew

doi:10.3855/jidc.8954

Abstract

The isolation of a rare community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strain from an infected dog bite wound is here reported. A 27-year old man presented with a deep open wound on his right hand caused by his dog's bite at the outpatient clinic of Infectious Disease Hospital (IDH), Kuwait. A wound swab was obtained for bacteriological culture and susceptibility testing. The wound culture yielded pure heavy growth of an MRSA isolate, designated IDH70, which was susceptible to vancomycin, teicoplanin, erythromycin, clindamycin, trimethoprim, fusidic acid and rifampicin. The patient was successfully treated with a combination of rifampicin and cotrimoxazole twice daily for 10 days. Molecular characterization revealed that IDH70 was positive for genes encoding Panton-Valentine leucocidin. IDH70 also carried the SCCmec V genetic element, belonged to coagulase type XIIIa, spa type t903, and was assigned to clonal complex 1153 and sequence type ST1153 (ST1153-V-t903). The report highlights the increasing burden of CA-MRSA in the community and the risk of its acquisition from bites of companion animals.

Highlights

Methicillin-resistant S. aureus (MRSA) is a growing global problem in human and veterinary medicine [1,2,3]
Antibiotic resistance is a growing problem among S. aureus isolates obtained from healthy or infected companion animals since they may act as reservoirs of antimicrobial-resistant bacteria that may be transferred to humans when they interact with the animals [10,11]
This paper reports the characteristics of a rare strain of community-acquired MRSA (CA-MRSA) isolated from infected soft tissue of a patient bitten by his dog

Summary

Introduction

Methicillin-resistant S. aureus (MRSA) is a growing global problem in human and veterinary medicine [1,2,3]. Close contact between household pets and humans through petting, licking or biting, offers favorable conditions for the transmission of pathogenic bacteria [5] Companion animals such as cats and dogs are frequently colonized by S. aureus including MRSA and can become infected [5,6,7]. Antibiotic resistance is a growing problem among S. aureus isolates obtained from healthy or infected companion animals since they may act as reservoirs of antimicrobial-resistant bacteria that may be transferred to humans when they interact with the animals [10,11]. Wound culture yielded pure growth of MRSA isolate, designated IDH70, which was susceptible to vancomycin, teicoplanin, erythromycin, clindamycin, trimethoprim, fusidic acid and rifampicin. DNA microarray using Identibac S. aureus genotyping kit 2 (Alere Technology, Jena, Germany) was employed to investigate virulence and antibiotic resistance genes according to the manufacturer’s instructions

Results

Discussion

Conclusion