Characterization of a blue-green pigment extracted from Pseudomonas aeruginosa and its application in textile and paper dyeing.

Abstract

Microorganisms are a promising source of colorants with large economic potential. Owing to better bio-degradability and higher eco-compatibility, microbial pigments propose promising avenues and can thus be a smart substitute for artificial pigments. The present work focused on the screening, isolation, and extraction of a blue-green pigment produced by soil microorganisms. The pigment-producing microorganism was identified as Pseudomonas aeruginosa on the basis of standard biochemical tests and by 16S rRNA sequencing. The purified blue pigment was characterized by high-performance liquid chromatography and gas chromatography-mass spectrometry. The antimicrobial activity of the microbial biocolor (3 × 108CFU/ml) was studied, and the zone of inhibition was found to be 10mm, 13mm, 9mm, and 7mm for E. coli, S. aureus, B. subtilis, and S. typhi, respectively. The evaluation of the biocolor as a dye was executed on different types of textiles and paper. The dyed fabrics were checked for washing, rubbing, and light and temperature fastness. Standard fabric properties of the fabrics dyed with the extracted microbial pigment were also assessed. The dyed fabrics were finally subjected to a patch test to check for any kind of allergic or hypersensitivity on human skin. The extracted pigment from Pseudomonas aeruginosa exhibited remarkable dyeing properties, indicating the scope for utilization of the pigment as a colorant on different types of textile and paper materials. The present study highlights the application of a bacterial pigment as a dyeing agent, which may raise its market value and probably replace toxic synthetic dyes due to its nontoxic nature, compatibility with various textiles, and cost-effectiveness.