Abstract
Inferior mechanical properties have always been a limitation of the bioresorbable membranes in GBR/GTR. This study is aimed at fabricating a bioresorbable magnesium-reinforced polylactic acid- (PLA-) integrated membrane and investigating its mechanical properties, degradation rate, and biocompatibility. The uncoated and fluoride-coated magnesium alloys, AZ91, were made into strips. Then, magnesium-reinforced PLA-integrated membrane was made through integration. PLA strips were used in the control group instead of magnesium strips. Specimens were cut into rectangular shape and immersed in Hank's Balanced Salt Solution (HBSS) at 37°C for 4, 8, and 12 d. The weight loss of the AZ91 strips was measured. Three-point bending tests were conducted before and after the immersion to determine the maximum load on specimens. Potentiodynamic polarization (PDP), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) were conducted on coated and uncoated AZ91 plates to examine corrosion resistance. Murine fibroblast and osteoblast cells were cultured on circular specimens and titanium disks for 1, 3, and 5 d. Thereafter, WST test was performed to examine cell proliferation. As a result, the coated and uncoated groups showed higher maximum loads than the control group at all time points. The weight loss of AZ91 strips used in the coated group was lower than that in the uncoated group. PDP, EIS, SEM, and EDS showed that the coated AZ91 had a better corrosion resistance than the uncoated AZ91. The cell proliferation test showed that the addition of AZ91 did not have an adverse effect on osteoblast cells. Conclusively, the magnesium-reinforced PLA-integrated membrane has excellent load capacity, corrosion resistance, cell affinity, and proper degradation rate. Moreover, it has great potential as a bioresorbable membrane in the GBR/GTR application.
Highlights
Periodontitis is a chronic inflammation of periodontal attachment tissues caused by local factors, which causes a destructive periodontal disease and alveolar bone loss [1, 2]
The results showed that the titanium membrane was equivalent to expanded PTFE (e-PTFE) membrane for periodontal tissue regeneration and can be used for the treatment of periodontal defects
Statistical analysis of the data was implemented by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc analysis, and a p < 0:05 was considered meaningful
Summary
Periodontitis is a chronic inflammation of periodontal attachment tissues caused by local factors, which causes a destructive periodontal disease and alveolar bone loss [1, 2]. The ISO selects an ideal biomaterial for GTR/GBR membranes, which should fulfill the main design criteria, such as biocompatibility, space-making, cell occlusiveness, tissue integration, and clinical operability [2, 4] These materials can be classified into nonbioresorbable and bioresorbable membranes, according to their degradation characteristics [4]. If the bioresorbable membrane material was absorbed too quickly, it would lead to the formation of an incomplete barrier membrane structure It means that the strength of the membrane material for surgeries was not enough to support the regeneration of periodontal tissues and bones [4, 10]. This study verified the feasibility of the design through mechanical experiments, degradation experiments, electrical corrosion tests, surface morphology, and elementary composition analysis for Mg and in vitro cell proliferation experiments, aiming to explore the effect of the application of the Mg-reinforced PLA composite membrane in GTR/GBR
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
