Abstract
Excretory/secretory (ES) products play an important role in the survival of Haemonchus contortus. Previously, a 66 kDa ES protein (P66) was identified that reacted with the infected animal sera and inhibited monocyte functions in vitro. In this study, the identity of P66 was established as glutamate dehydrogenase by mass spectrometry followed by enzymatic assay. This facilitated the generation of recombinant P66. The native and recombinant forms of the enzyme stimulated the proliferation of caprine peripheral blood mononuclear cells in vitro and also induced the production of cytokines IL-4 and IFN-γ, analogous to the immune response (Th1 and Th2), observed during natural infection. However, P66 showed no complement-inhibiting activity. The importance of P66 in the host–parasite relationship is discussed.
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Schedule a callMore From: Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
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