Abstract
Next-generation sequencing technologies provide an excellent opportunity to mining large numbers of EST-SSR markers quickly and cost-effectively for non-model organisms. Here we isolated and characterized 62 polymorphic EST-SSR markers in the blood clam, Tegillarca granosa, from the ESTs generated by 454 sequencing. The number of alleles for the 62 SSR markers in 25 individuals varied from 2 to 9, with an average of 3.90 alleles per locus. The observed heterozygosity ranged from 0.040 to 1.000, while the expected heterozygosity varied from 0.040 to 0.844. Polymorphic information content ranged from 0.038 to 0.806. Interspecific transferability of the 62 markers revealed that 16 were polymorphic in Scapharca subcrenata, resulting in a transferability rate of 25.81 %. To our knowledge, this is the first report on the survey of SSR transferability in blood clams. These novel polymorphic EST-SSR markers should be particularly useful for further investigation of population and conservation genetics.
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