Abstract
We characterized 36 new microsatellite markers for splittail (Pogonichthys macrolepidotus), an estuarine fish species endemic to the San Francisco Estuary. Genetic variation was assessed using 25 individuals from the Sacramento River and 16 individuals from the Petaluma River. Number of alleles per locus varies considerably, ranging from 3 to 19. One locus deviated significantly from Hardy–Weinberg equilibrium and a single pair of loci was found to be in linkage disequilibrium. Twenty-four markers successfully cross-amplified and were polymorphic in at least one of the five additional California cyprinid species (Ptychocheilus grandis, Siphateles bicolor, Lavinia exilicauda, Orthodon microlepidotus & Mylopharodon conocephalus) examined in this study.
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