Characterization of 28S rRNA sequences of cestoda parasite Electrotaenia malapteruri Fritsch, 1886 from the Electric catfish Malapterurus electricus (Siluriformes: Malapteruridae)

Abstract

Proteocephalids are cestoda parasites that mostly infect freshwater fish. The present study was carried out to investigate the presence of proteocephalids infecting the electric catfish Malapterurus electricus from Lake Manzala, Kafr El-Sheikh Governorate, Egypt. Morphological characterization revealed the present parasite is a cestoda belonging to the genus Electrotaenia. Morphologically, the recovered worms were characterized by an elongated body measuring 100–127 (120 ± 2) mm long and 0.92–2.11 (2.76 ± 0.1) mm wide. The anterior part of the worm was obvious terminated at a spherical scolex measured 1.12–1.91 (1.72 ± 0.01) mm long and 1.12–1.65 (1.42 ± 0.01) mm wide with a rostellum-like apical organ equipped by 5–6 irregular rows of minute hooklets, as well as four uniloculate suckers with a diameter of 0.13–0.15 (0.14 ± 0.01) mm and covered with microtriches. A long unsegmented neck was observed followed by acraspedote and anapolytic strobila consisted of 85–120 proglottids divided into 50–58 immature, 12–19 mature, and up to 49 gravid proglottids. Molecular characterization based on 28S rRNA sequences was done to confirm the taxonomy of this parasite based on its morphology. It was observed that there was a close identity up to 72.0% with other protocephalid species obtained for comparison from the GenBank. Also, the data obtained revealed that there was high blast scores and low divergence between the present parasite and previously described Electrotaenia malapteruri (acc. no. JX477434). Phylogenetic analysis showed that the parasite sequence in conjunction with existing data investigates the placement of this protocephalid species within Proteocephalidea. It was shown that the present species is deeply embedded in the genus Electrotaenia with close relationships to other Electrotaenia malapteruri as a putative sister taxon.