Characterization of 19 polymorphic microsatellite markers for Asian yellow pond turtle (Mauremys mutica)

Abstract

Asian yellow pond turtle (Mauremys mutica) is an endangered species. In this study, we developed and characterized 19 novel polymorphic microsatellite markers for M. mutica through the Restriction-site Associated DNA tags sequencing (RAD-seq). 60 individuals were used to assess the level of polymorphism of each locus. The number of alleles ranged from 4 to 25, the observed and expected heterozygosity ranged from 0.40000 to 0.90000 and 0.39572 to 0.93201, respectively. These markers would be used in genetic studies helping conservation and management of M. mutica.