CHARACTERIZATION, FTIR SPECTRA PROFILE AND PLATELET ANTI-AGGREGATION ACTIVITY OF CRUDE FUCOIDAN FROM SARGASSUM CRASSIFOLIUM

Abstract

Objective: This research was aimed to investigate the characterization and FTIR spectra profile in correlation with platelet anti-aggregation activity of crude fucoidan from Sargassum crassifolium.
 Methods: Dried brown seaweed powder was extracted using 0.1 M of HCl solution after pre-macerated with 85% of ethanol, precipitated with ethanol, and lyophilized as crude fucoidan. It was analyzed for fucose, sulfate, carbohydrate content, FTIR spectra and evaluated the in vitro platelet anti-aggregation activity. Partial least square (PLS) analysis was conducted to identify the functional group that contributes to the platelet anti-aggregation activity.
 Results: The fucose, sulfate, and carbohydrate content were 3.64-9.44%, 12.05-18.01%, and 11.45–21.41%, respectively. The results of the statistical analysis showed significant differences in fucose, sulfate, carbohydrate content, and platelet anti-aggregation activity of crude fucoidan extracted from Sargassum crassifolium at different harvest times. According to the result of PLS analysis using FTIR spectra data and the value of IC50, the functional group that contributed to the platelet anti-aggregation activity were OH, C=O, and S=O.
 Conclusion: Platelet anti-aggregation activity of crude fucoidan was proportional to the level of sulfate not to fucose and carbohydrates levels. The functional group of crude fucoidan which were correlated with this activity could be identified.