Characterization extrinsic factors of immobilized cells thermoxilanolytic bacteria in producing xylanase

Abstract

Enzyme is one of important commodity in industrial world. The majority of industrial enzymes are produced by microorganisms through fermentation. The use of enzymes is largely as a biocatalyst in chemical transformations. Currently there are about 400 known enzymes and about 200 are used commercially. The enzyme industry has grown rapidly and occupies an important position in the field of industry one of which is xylanase enzyme. Xylanase can be produced by molds and bacteria through a fermentation process. Groups of bacteria that are known to produce xylanase are Bacillus and Clostridium. In today’s microbial use of thermophilic bacteria has commercial value because it is able to produce stability of heat-resistant enzymes. In producing enzymes cell immobilization techniques are used to facilitate the purification of products, increase productivity and ease in controlling cell stability. The aim of this study was to Characterization Extrinsic Factors of Immobilized Cells Thermoxilanolytic Bacteria in Producing Xylanase. This research was conducted in June 2017- June 2018 in Laboratory of Microbiology FMIPA UNP. This research was using descriptive method. This research conclusion that temperature effect on xylanase activity with optimum temperature was 70°C and pH affects the xylanase activity with the highest pH was 7.5.