Abstract
This article contains original data, figures and methods used in the characterization of the liposomal carrier ‘DDC642’ for topical applications, described in “An elastic liposomal formulation for RNAi-based topical treatment of skin disorders: proof-of-concept in the treatment of psoriasis” (Desmet et al., 2016) [1]. Several elastic liposomal formulations have been evaluated for their ability to encapsulate and deliver RNA interference (RNAi) molecules to cultured primary skin cells. The efficiency and effectiveness of these liposomes were compared to that of our previously characterized liposomes, the ‘SECosomes’ (SEC) (Geusens et al., 2010) [2]. After selection of a potential superior carrier, based on encapsulation and transfection efficiency data (Desmet et al., 2016) [1], the selected DDC642 liposomes were characterized more in-depth. Herein, a detailed characterization of the DDC642 liposome and RNAi-loaded lipoplexes is given, including the matching protocols.
Highlights
This article contains original data, figures and methods used in the characterization of the liposomal carrier ‘DDC642’ for topical applications, described in “An elastic liposomal formulation for RNA interference (RNAi)-based topical treatment of skin disorders: proof-of-concept in the treatment of psoriasis” (Desmet et al, 2016) [1]
Several elastic liposomal formulations have been evaluated for their ability to encapsulate and deliver RNA interference (RNAi) molecules to cultured primary skin cells
The RNAi encapsulation efficiency has been analyzed for several liposomal formulations. (Psoriasis-induced) keratinocytes and melanocytes were cultured in vitro prior to transfection with empty liposomes or loaded lipoplexes, to assess respectively cytotoxicity and transfection efficiency
Summary
The RNAi encapsulation efficiency has been analyzed for several liposomal formulations. (Psoriasis-induced) keratinocytes and melanocytes were cultured in vitro prior to transfection with empty liposomes or loaded lipoplexes, to assess respectively cytotoxicity and transfection efficiency. Cryo-TEM, CellTiter-Glo assay and dynamic light scattering was used to assess the morphology, cytotoxicity and particle size of the (loaded) liposomes, respectively. The data provide a more thorough characterization of the DDC642 liposomes, which hold great potential as topical delivery system. The DDC642 carrier was evaluated for its potential as topical RNAi delivery system by measuring the encapsulation efficiency, transfection efficiency and efficacy in vitro. Data is provided on the cytotoxicity of the empty liposomes and physicochemical and biological stability of the RNAi-loaded lipoplexes. Morphological data on the DDC642 liposomes and lipoplexes is provided as Cryo-TEM images
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