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Abstract
ABSTRACTCharacterization, antioxidant activities and hepatoprotective effects of polysaccharides from pre-pressing separation apple (Fuji) peel pomace were investigated in this study. Gel permeation chromatography indicated that apple peel polysaccharides (APPs) were a kind of heteropolysaccharides composed of three fractions with homogenous polarity. Both GC–MS and HPLC analysis showed that arabinose, galactose and galacturonic acid were the main monosaccharides in APP. In vitro antioxidant activities of APP were characterized. Furthermore, administration of APP (450 mg/kg bw) in mice significantly decreased the CCl4-induced elevations of serum alanine aminotransferase, asparate aminotransferase and lactic dehydrogenase activities, and hepatic malondialdehyde level and inhibited the decreases in superoxide dismutase and glutathione peroxidase activities caused by CCl4. Histopathological observation further confirmed that APP could protect the liver tissues from CCl4-induced histological alternation. These findings indicate that APP has the antioxidant and hepatoprotective potentials and provide a scientific basis for comprehensive utilization of pre-pressing separation apple peel pomace.
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