Abstract
Lignin peroxidase (LiP) and manganese peroxidase (MnP) purified from the culture filtrate of the basidiomycetes Bjerkandera adusta catalyzed the oxidation of various phenolics and aromatic amines in the reaction mixture containing water-miscible organic solvents such as ethylene glycol, methylcellosolve and acetone. Aromatic compounds that have low ionization potential and high hydrophobicity were found to be preferable substrates of the enzymes in these solvents. Parameters that characterize the activity of LiP and MnP in organic solvents were analyzed. By using MnP, coniferyl and sinapyl alcohols were polymerized to c-DHP and s-DHP, respectively, with a high yield in the mixed solvent of acetone and methylcellosolve. NMR analysis showed s-DHP synthesized by MnP contained higher content of β-O-4 substructure than the DHP synthesized by horseradish peroxidase. Using this reaction, polymerization products were obtained from various phenols, aromatic amines and other compounds in the media containing acetone or dioxan.
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