Abstract
φX174 protein synthesis in ultraviolet-irradiated Escherichia coli C hcr − cells and. unirradiated cells was examined on sodium dodecyl sulfate-acrylamide gels. Eight viral proteins were identified and six of these assigned to cistrons. Viral protein synthesis in ultraviolet-irradiated cells is not normal. There is a large accumulation of coat protein and relatively little synthesis of cistron V (single-strand DNA synthesis) protein. The relative amount of the other φX gene products synthesized is also different from that observed during a normal infection of an unirradiated cell.
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