Characterization and symbiotic evalution of rhizobium bacteria from various plants on soybean (Glycine Max L) plants in green house

Abstract

Rhizobium bacteria are bacteria including Plant Growth Promoting Rhizobacteria (PGPR) that are able to fix nitrogen, solubilise phosphate, produce enzymes of protease, IAA hormone and siderophore so that it plays, an important role in increasing agricultural productivity. Several rhizobia have been successfully isolated from 8 legume plants from various regions, with the aim of obtaining Rhizobium isolates which are potential, effective and efficient as biological fertilizer agents. A total of 8 isolates were successfully isolated using selective media for Rhizobium bacteria, namely YEMA, following their activities were tested qualitatively N fixing, solubilise phosphate, protease, IAA hormone and siderophore production. All isolates thrive on YEMA+CR media and showed fast growth in YEMA + BTB.. A total of 11 inoculation treatments were tested on soybean plants consisting of 1. EKP (3), 2. EKP (4), 3. i (1), 4 (combined 1-3), 5. 1 (2), 6. B (1), 7. H (2), 8 ( combined 5-7), 9. A (2), 10.2 (1), 11 (combined 9-10). As a control plant without inoculation and without N fertilizer (K1) and plants without inoculation and with N fertilizer equivalent to 100 kg/ha (K2). The planting media used for greenhouse experiments were sterile sand media. The experimental design used were a completely randomized design with 3 replications. Plants growth were observed with parameters included plant height and number of leaves (at 1,2,3,4,5,6 and 7 weeks). Soybean plants werw harvested at 50 days after planting, following measurement of dry weight of canopy, roots, root nodules, total plants and chlorophyll content. The results showed that 8 individual isolates and 3 combined isolates inoculated to soybean plants varied, all of which were able to increase growth. Isolates number H(2) and 1(2) (isolates of nodules root of Peanut plants) gave the highest yields on the growth of soybean plants.