Abstract
A hexasaccharide containing sialic acid has been isolated from the urine of pregnant women. It contains d-galactose, d-glucose, N-acetyl- d-glucosamine, and sialic acid in the proportions of 2:1:1:2. Its structure, established by methylation analysis, enzymic digestion, and potassium borohydride reduction, is that of lacto- N-tetraose with one of the residues of sialic acid linked α-(2→3) to the terminal nonreducing residue of d-galactose, and the other sialic acid residue linked α(2→6) to the N-acetyl- d-glucosamine residue. This hexasaccharide is related to human pregnancy, as it has not been found in nonpregnant, normal-woman urine and is present in human milk.
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