Characterization and Some Cultural Conditions of a Cyclodextrin Glycosyltransferase-producing AlkalophilicBacillussp.

Abstract

A cyclodextrin glycosyltransferase-producing bacterium was isolated from soil in a high alkaline pH medium containing 1% of Na2CO3. The isolated alkalophilic microorganism was identified as a member of the genus Bacillus. Highest yield of the enzyme was obtained by using the following medium; 1% soluble starch, 5% corn steep liquor, 0.1% K2HPO4, 0.02% MgSO4·7H2O and 1% Na2CO3. The crude enzyme showed a very broad pH-activity curve and had two optimum pH ranges at 4.5-5.0 and 7.5-8.5. The crude enzyme also catalyzed the formation of cyclodextrins at both of the optimum pH ranges.