Abstract
The characterization and selection of molecular markers are important for genetic pre-breeding programs since they make it possible to choose the most appropriate markers to be used in future research. Therefore, enabling the generation of subsidies for genetic-molecular studies in algabora (Prosopis juliflora (Sw.) DC). The amplification profile was characterized. It was generated from 17 pairs of RGA primers (Resistance Gene Analogs) in 20 samples of genomic DNA of P. juliflora extracted from specimens collected in the city of Itapetinga, Bahia. The amplifications were performed according to previously published laboratory routines and the amplification profiles analyzed from the photodocumentation of the electrophoresis results in 2% agarose gels. Based on the amplification profiles the primer pairs were classified as: Suitable: amplifications in the whole samples and with easy visualization; Reasonable: amplification in parts of the samples and/or difficult to visualize or Inadequate: absence of visible amplification products. Descriptive analyzes associated with the number of generated markers, percentage of polymorphism, expected heterozygosity (He) and the content of polymorphic information (PIC) were also performed. In a nutshell, 12 out of the 17 pairs of RGA primers generated amplification products with easy visualization and only two of these 12 pairs of primers were monomorphic. The percentage of polymorphism varied from 60% to 100%, He and PIC presented an average of 0.21 (ranging from 0 to 0.38) and 0.17 (ranging from 0 to 0.29), respectively. The results confirm that the RGA primers present adequate characteristics for genetic studies in P. juliflora, making it possible to prioritize 12 pairs of primers, which are subject to genetic improvement studies.
Highlights
The species Prosopis juliflora (Sw.) DC, popularly known as algaroba, belongs to the family Fabaceae, subfamily Mimosoideae
The genetic diversity study of Prosopis cineraria L. and Prosopis juliflora (Sw.) DC L. using ISSR and RAPD markers is an example of research of this nature that contributes to the understanding of the intra-specific diversity in the genus Prosopis (Elmeer & Almalki, 2011)
Among the molecular markers used in genetic studies of plants are the RGA (Resistance Gene Analogs)
Summary
The species Prosopis juliflora (Sw.) DC, popularly known as algaroba, belongs to the family Fabaceae, subfamily Mimosoideae. The crucial step, after identification and collection of the sample population, is the definition of the molecular markers to be used in it In this context, the characterization and selection of primers contribute to cost reduction and to optimize the time spent before the evaluation of the entire population, avoiding the use of primers unsuitable for the target species (Silva, Freitas, Santos, Cardoso, & Cerqueira-Silva, 2018). Among the molecular markers used in genetic studies of plants are the RGA (Resistance Gene Analogs) These are markers associated with regions considered rich in leucine and nucleotide binding sites (Nucleotide Binding Sit Leucine Rich Repeat - NBS-LRR). Considering the present context, the objective was to characterize the amplification profile of different combinations of RGA primers and to select the most appropriate combinations to support genetic studies in Prosopis juliflora (Sw.) DC
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