Abstract
Being a key industrial enzyme, tannase is extensively applied in various fields. Despite the characterizations of a large number of tannases, there are hardly a few tannases with exceptional thermostability. In this detailed study, a tannase-encoding gene named tanA was identified from Aureobasidium melanogenum T9 and heterologously expressed in Yarrowia lipolytica host of food grade. The purified tannase TanA with a molecular weight of above 63.0 kDa displayed a specific activity of 941.4 U/mg. Moreover, TanA showed optimum activity at 60°C and pH 6.0. Interestingly, TanA exhibited up to 61.3% activity after incubation for 12 h at 55°C, signifying its thermophilic property and distinguished thermostability. Additionally, TanA was a multifunctional tannase with high specific activities to catalyze the degradation of various gallic acid esters. Therefore, this study presents a novel tannase, TanA, with remarkable properties, posing as a potential candidate for food and agricultural processing.
Highlights
As a type of polyphenol compound generated to resist a bad growth environment, tannins are extensively present in higher plants (Chung et al, 1998)
The previous study of the genes related to tannin degradation in A. melanogenum T9 has suggested that there existed several tannases with different molecular masses, amino acid sequences, gene expression patterns, and diverse properties, which are together
The tannases from different categories are often produced in the same fungal species due to species-specific accumulative effects and the impacts of exterior circumstances
Summary
As a type of polyphenol compound generated to resist a bad growth environment, tannins are extensively present in higher plants (Chung et al, 1998). The formation of stable complexes with various biological macromolecules, such as polysaccharides and proteins, provides lower nutritive value to food, making them nutritionally undesirable (Pan et al, 2020). Tannins are one of the primary causes of low food intake, slow growth, low level of fodder utilization rate, and low protein breakdown in laboratory animals (Becker and Makkar, 1999; Min et al, 2005; Sengil and Oezacar, 2009). There still exist some special microorganisms which can grow and reproduce stably in an environment containing tannins, making the full utmost of the secreted enzymes to decompose tannins into small-molecule phenolic compounds (Chhokar et al, 2013).
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