Abstract
Nine bacterial strains were isolated from Thai fermented fish by the standard dilution technique using JCM no. 377 medium. The rod shaped isolate FN2-3 was identified as Virgibacillus dokdonensis while isolates FN2-3 and FN6-6 as V. halodenitrificans, FN1-13 as Corynebacterium variabile, FN1-10 as Oceanobacillus iheyensis and FN3-7 was Bacillus amyloliquefaciens subsp. plantarum. The coccal isolates, FN6-1 and FN6-7 were Staphylococcus saprophyticus subsp. bovis and FN6-8 was S. saprophyticus subsp. saprophyticus, based on their phenotypic characteristics and 16S rRNA gene sequence analyses at a 99.63-100% sequence similarity. Their lipase activity in complex medium (CM), CM medium with 1% (v/v) Tween 20 or CM medium with 1% (v/v) Tween 80 ranged from 1.12 ± 0.03-3.77 ± 0.04 unit/mL, with the highest lipase activity found with V. dokdonensis FN1-8 cultivated with CM with 1% (v/v) Tween 80.
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