Characterization and screening of anti-melanogenesis and anti-photoaging activity of different enzyme-assisted polysaccharide extracts from Portulaca oleracea L.

Xingyu Tao,Xuan Hu,Tongchuan Wu,Dongyue Zhou,Di Yang,Xue Li,Yunhua Fu,Fei Zheng,Hao Yue,Yulin Dai

doi:10.1016/j.phymed.2023.154879

Abstract

BackgroundThe flavonoids and polysaccharides in Portulaca oleracea L. (PO) have significant antibacterial and antioxidant effects, which can inhibit common bacteria and remove free radicals in the body. However, there was little research on the use of PO to alleviate hyperpigmentation and photoaging damage. PurposeThis study was to investigate the anti-photoaging and whitening activity mechanism of polysaccharide of PO (POP) in vitro and in vivo. MethodIn this study, 16 fractions obtained by four enzyme-assisted extraction from PO and their scavenging capabilities against 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals were evaluated. Among these fractions, a polysaccharide fraction (VPOP3) showed the strongest biological activity. VPOP3 was characterized by Fourier-transform infrared spectroscopy, molecular weight (MW), and monosaccharide composition analysis, and the protective effect of VPOP3 on photoaging and hyperpigmentation was researched. ResultsVPOP3 is a low-MW acidic heteropolysaccharide with MW mainly distributed around 0.71KDa, arabinose as its main monosaccharide component. VPOP3 reliably reduced the reactive oxygen species levels in cells and zebrafish and the level of lipid peroxidation in zebrafish. In addition, VPOP3 inhibited UVB-induced apoptotic body formation and apoptosis by downregulating caspase-3 and Bax and upregulating Bcl-2 in mitochondrion-mediated signaling pathways. On the other hand, VPOP3 at high concentrations significantly downregulated the expression of microphthalmia-associated transcription factor, tyrosinase (TYR), and TYR-related protein-1 and TYR-related protein-2 in the melanogenic signaling pathway to achieve a whitening effect. ConclusionThe above results showed that VPOP3 has superior activities of anti-photoaging and anti-melanogenesis and can be utilized as a safe resource in the manufacture of cosmetics.

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