Abstract
Canine hemolysates exhibited two minor and one major hemoglobins when chromatographed on Bio Rex-70 column with 0.05 M phosphate-0.01 M KCN (pH 6.5) containing 0.015 M NaCl. The first minor fraction was electrophoretically heterogeneous, constituting at least two hemoglobins. The second was completely homogeneous with somewhat faster anodal mobility than the major one and the difference was shown to be localized solely in the beta subunits. Thiobarbituric acid reaction was strongly positive for this hemoglobin, indicating the presence of glycosyl ketoamine linkage. We describe a simple and reliable procedure for quantitation of the canine glycosylated hemoglobins with a home-made Bio Rex-70 mini-column.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
