Abstract
The amino acid compositions of the high relative molecular mass(Mr) or high molecular weight (HMW) subunits of wheat glutenin were determined by the phenylthiocarbamyl (PTC) derivative procedure with polypeptides separated by sodium-dodecyl sulphate polyacrylamide gel electrophoresis SDS-PAGE and electroblotted onto polyvinylidene difluoride (PVDF) membranes. Four bands (three corresponding each to a single subunit and one to a mixture of two subunits) from the cultivar Courtot were analysed, either directly on the electroblotted membrane or after elution of the polypeptides. As little as 10 μg of protein was suitable for a complete analysis. The compositions obtained were consistent with published data obtained either from similar biochemical studies or from cDNA sequences when available.
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