Characterization and proteome analysis of the extracellular vesicles of Phytophthora capsici

Abstract

Extracellular vesicles (EVs) are important for the transport of biomolecular materials and intercellular communication in eukaryotes. Recent research has revealed that they are involved in plant–pathogen interaction and pathogenesis of infected cells. Phytophthora capsici is a highly devastating oomycete pathogen with a broad host range. To increase infection and facilitate colonization, it secretes effector proteins during interaction with plants. In this study, we characterize for the first time the EVs from pathogen P. capsici through transmission electron microscopy. For the biological study of EVs, results showed that mixing high concentrations of EVs with zoospores could enhance the virulence of P. capsici. By sequencing the protein composition of EVs by liquid chromatography in tandem with mass spectrometry we found that there are many proteins related to metabolism, oxidation/reduction, and transport in EVs, indicating that they have important roles in pathogenesis and immunological processes within the host. SignificanceExtracellular vesicles (EVs) are important both at normal physiological processes as well as pathological progression during pathogen and host interaction. In this paper we first establish the extraction method of EVs from the important oomycete pathogen Phytophthora capsici. Bioinformatics analysis of EV proteomics revealed a variety of pathogenic-related proteins, like oxidation/reduction-related proteins, stress response proteins as well as elicitors. Our results will help better understanding the biological function of the EVs during plant and P. capsici interaction and providing the evidence for the role of EVs in pathogenesis of the P. capsici.