Abstract
Gedi (Abelmoschus Manihot L.) is a tropical plant in the Malvaceae family, one of the groups of plants in the herbal community. Gedi plants are known to contain flavonoids, hormones, alkaloids, tannins and phenolic compounds. An ultrasonic extraction tool is used in the extraction process of Gedi leaves. The objective of the study is to know the yield and analyze Gedi leaf extract, including proximate analysis and active substance components with LCMS-QTof. The material used is Gedi leaves from Manado. Ultrasonic extraction was carried out with a dry Gedi leaf ratio and Ethanol 1:40 (m/v) with a time of 30 minutes. The concentration of the extract is with a rotary evaporator temperature of 50-60℃. The yield of the extract of Gedi leaf is 9.86%. Study of the proximity of dried Gedi leaf powder revealed 27.135% protein, 41.675% carbohydrate, 329 kcal energy, 11.235% water and 13.83% ash material. Meanwhile for proximity products, the Gedi leaf extract contains 0.635% protein, 1.27% carbohydrate, 9.26% total energy, 7.1% water, and 0.635% ash. The active compounds in the Gedi extract have been analyzed using LCMS. Trigonelline, Gentiatibetine, Harman, Periplocoside C, and Biotin were detected using the positive-ionization mode, whereas Chloramphenicol was detected with negative-ionization mode.
Highlights
Gedi (Abelmoschus Manihot L.) is a tropical plant of the Malvaceae family and is classified as a medicinal herbal plant [1,2,3]
It reported that proteins and carbohydrates in Gedi leaf extract made up 9.6% and 17.76% respectively, compared to 6.05% of fats [1]
Could be expected that Gedi leaf extraction using ethanol would result in higher yields than that using sequential solvents
Summary
Gedi (Abelmoschus Manihot L.) is a tropical plant of the Malvaceae family and is classified as a medicinal herbal plant [1,2,3]. A. Manihot may be used as an alternative medicine to lower blood glucose levels and blood pressure and to act as an anti-inflammatory, antioxidant and antidepressant [4,5,6,7,8]. It is known to contain flavonoids, steroids, tannins and phenols. The study conducted by Pine et al (2010) showed that Gedi leaves had relatively high concentrations of flavonoids (23-41 %) which made it a good candidate as a source of antioxidants [5]. Tannin is a compound of polyphenols[7]
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