Abstract
Background Currently, stirred tank reactors (STR) represent the gold standard for the large scale growth of suspension cell lines. Culture performance is strongly influenced by the efficiency of mixing, as measured by the mass transfer coefficient and mixing time. The success of traditional stainless steel or glass STR systems lies in their impeller driven agitation that efficiently mixes large volumes of culture fluid. However, the transition from stainless and glass STR vessels to single use STR systems still remains a challenge, particularly in terms of replicating efficient mixing. Many single use STRs use magnetically coupled driving mechanism to eliminate the risks of biocontainer leaks from rotating mechanical seals. While this design mitigates the risks of compromising sterility, insufficient magnet strength limits the power that can be transmitted to the culture fluid, and often results in poor mixing and mass transfer performance.
Highlights
The benefits of single-use technologies in both upstream and downstream operations are widely acknowledged by the biopharmaceutical industry, and have resulted in radical changes to the design and operation of many pharmaceutical processes
To achieve the same or better performance from a single-use bioreactor presents a significant challenge to product designers and developers
Published: 14 December 2015 doi:10.1186/1753-6561-9-S9-P60 Cite this article as: Irfan et al.: Characterization and performance of the AllegroTM stirred tank reactors (STRs) 1000 single-use stirred tank bioreactor system
Summary
The benefits of single-use technologies in both upstream and downstream operations are widely acknowledged by the biopharmaceutical industry, and have resulted in radical changes to the design and operation of many pharmaceutical processes. Multi-use, cleanable glass or stainless steel stirred tank reactors (STRs) have been used successfully for growth of suspended cell lines for both small and large scale systems. To achieve the same or better performance from a single-use bioreactor presents a significant challenge to product designers and developers. The poster describes the studies aimed at characterizing the fluid dynamics of the Allegro STR 1000 bioreactor by measurement and modelling of specific physical parameters known to be critical for mammalian cell culture:. Oxygen transfer rates, mixing times and carbon dioxide stripping rate
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