Abstract
Microbial degradation of keratinous wastes is preferred over physicochemical methods, because it’s a cost effective and eco-friendly. Novel habitats are promising for discovery of new microbial strains. Towards isolate a keratinolytic bacteria, screening of bacterial strains from a poultry farm soil in Namakal district, Tamil Nadu, India was done and a promising the isolate C3 was found to degrade native chicken feather efficiently. It could grow over a pH range 7 at 40°C and in presence of potassium nitrate as a nitrogen source and citrate as a carbon source. Based on phenotypic characterization and 16S rRNA gene sequence analysis, the keratinolytic isolate was identified as Pseudomonas stutzeri C3. It produced 30.86±1.08 U/ml keratinase in raw feather medium and complete feather degradation at 5 days incubation periods.
Highlights
Keratin is the most abundant protein in epithelial cells and forms major components of skin, hair, nail, feather and wool
The nutritional upgrading of feather meal with the treatment of microbial keratinase might lead to a significant increase in the availability of amino acids in feather keratin
The present study aims at the degradation of feather by keratinase produced by Pseudomonas stutzeri C3, a keratinolytic bacteria isolated from poultry farm waste site and the optimization of keratinase production
Summary
Keratin is the most abundant protein in epithelial cells and forms major components of skin, hair, nail, feather and wool. Disposal of feather waste is a major problem because simple dumping in the ground leads to the soil pollution and burning it adds to the SO2 and CO2 content in the environment and causes air pollution. This mammoth size of discarded feather, apart from polluting the soil or air, causes various human ailments including chlorsis, mycoplasma and fowl cholera (Revathi et al, 2013). The nutritional upgrading of feather meal with the treatment of microbial keratinase might lead to a significant increase in the availability of amino acids in feather keratin. The present study aims at the degradation of feather by keratinase produced by Pseudomonas stutzeri C3, a keratinolytic bacteria isolated from poultry farm waste site and the optimization of keratinase production
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