Characterization and Optimization of Alkaline Protease Production from Bacillus licheniformis HSW-16 Isolated from Sambhar Salt Lake

Raju Singh ,Pallavi Jha

doi:10.3126/ijasbt.v3i2.12757

Abstract

Halophilic microorganisms are recognized as potential source of secondary metabolites including enzymes and drugs with wide agricultural and industrial applications. In the present study protease producing halotolerant bacterium Bacillus licheniformis HSW-16 was isolated from hypersaline Sambhar lake, Rajasthan India. Protease production was performed by using azocasein as substrate. Confirmation of protease production was also done by amplification of alkaline protease gene and sequencing. The various nutritional factors such as carbon and nitrogen source and other physiological parameters like pH, temperature, incubation time and agitation speed were optimized for optimum protease production. The enzyme was active in pH range 7-10, temperature 25 °C-40 °C and salt concentration of 1.5M. The characteristics demonstrated by this isolate showed that it could be used as a potential source of enzyme.Int J Appl Sci Biotechnol, Vol 3(2): 347-351 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12757

Highlights

Proteases belong to the class of hydrolytic enzymes that cleave peptide bonds between amino acid residues, and are the most important commercially used enzymes in the world
Protease being extensively prevalent in the environment, the most ideal source of obtaining them is from microbes because of their rapid growth, limited space required for their cultivation and ease of genetic manipulation to make new enzymes with desirable properties for various applications (Kocher and Mishra, 2009)
Isolation and identification of strain A halophilic bacteria Bacillus licheniformis was isolated from Sambhar salt lake and screened for protease production on casein agar plate for protease production

Summary

Introduction

Proteases belong to the class of hydrolytic enzymes that cleave peptide bonds between amino acid residues, and are the most important commercially used enzymes in the world They constitute two-thirds of the total enzymes used in various industries and commercially exploited in the detergent, leather, food, pharmaceutical, diagnostics and various other chemical industries (Oberoi et al, 2001). Among them alkaline proteases are of particular interest due to their potent applications in the detergent industry as a cleaning additive in the detergent formulation (Gupta, 2003) Though several microorganisms such as fungi, yeast are known to produce alkaline proteases, microbial proteases especially from Bacillus sp. To compensate the industrial demand for an alkaline protease possessing properties to overcome these challenges, there is a constant need to search for new enzyme sources

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Conclusion