Characterization and mechanism analysis of tylosin biodegradation and simultaneous ammonia nitrogen removal with strain Klebsiella pneumoniae TN-1

Abstract

A novel bacterial strain that exhibited a high capacity for the simultaneous degradation and removal of tylosin and ammonia nitrogen, respectively, was isolated from tylosin fermentation dregs (TFDs) and identified as Klebsiella pneumoniae TN-1. The removal efficiencies of tylosin and ammonia nitrogen reached 95.31% and 83.26%, respectively, at initial concentrations of 300 mg/L for both. Three identified intermediates with less toxicity indicated that de-sugarization and hydrolysis were the proposed biodegradation pathways. The results also suggested that strain TN-1 could reduce nitrogen loss by transforming ammonium into nitrate nitrogen according to the transcriptional expression of nitrogen transformation-related genes and the activities of functional enzymes. Moreover, strain TN-1 effectively reduced ammonia volatilization by 65.20% and facilitated tylosin degradation, with a maximum removal efficiency of 57.35% in the simulated fermentation process of TFDs. This work provides an efficient bioaugmentation for simultaneous antibiotic degradation and nitrogen conservation during the composting process.