Abstract
As the last stage of plant development, leaf senescence has a great impact on plant’s life cycle. Genetic manipulation of leaf senescence has been used as an efficient approach in improving the yield and quality of crop plants. Here we describe an ethyl methane sulfonate (EMS) mutagenesis induced premature leaf senescence mutant yellow leaf 1 (yl1) in common tobacco (Nicotiana tabacum L.). The yl1 plants displayed early leaf yellowing. Physiological parameters and marker genes expression indicated that the yl1 phenotype was caused by premature leaf senescence. Genetic analyses indicated that the yl1 phenotype was controlled by a single recessive gene that was subsequently mapped to a specific interval of tobacco linkage group 11 using simple sequence repeat (SSR) markers. Exogenous plant hormone treatments of leaves showed that the yl1 mutant was more sensitive to ethylene and jasmonic acid than the wild type. No similar tobacco premature leaf senescence mutants have been reported. This study laid a foundation for finding the gene controlling the mutation phenotype and revealing the molecular regulation mechanism of tobacco leaf senescence in the next stage.
Highlights
IntroductionTobacco (Nicotiana tabacum L.) is an important agricultural crop widely grown in the world
Tobacco (Nicotiana tabacum L.) is an important agricultural crop widely grown in the world.It plays an important role in plant genetics and transgenic research as a model plant [1,2]
Plant leaf senescence regulation has been extensively studied in model systems such as Arabidopsis and rice, limited information is available about tobacco leaf senescence regulation at present
Summary
Tobacco (Nicotiana tabacum L.) is an important agricultural crop widely grown in the world. It plays an important role in plant genetics and transgenic research as a model plant [1,2]. It can be used as a bioreactor for vaccine production [3,4,5]. Studying tobacco leaf senescence has important theoretical significance and a practical application value. Several studies focused on transcriptome and metabolome of tobacco leaf senescence [9,10,11,12]
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