Abstract
Plasma membranes have been isolated from grape leaves using a two-phase aqueous polymer technique which differed from the usual procedure in order to counter difficulties posed by the fibrous nature and high starch content of this material. The lipid composition (sterols, phospholipids + sphingolipids and their fatty acids) of the purified plasma membrane was determined and compared with that of the microsomal fraction (100 000 g). The major differences between the membrane and microsomal fractions were increases in the proportions of stearic acid, phosphatidylcholine and, to a lesser extent, phosphatidylethanolamine and a decrease in linolenic acid. Phosphatidylglycerol and diphosphatidylglycerol were present in the microsomal fraction, but not in the plasma membrane. Unusually large amounts of cerebrosides (compared with some other plant species) were found in both membrane fractions. The lipid composition of the plasma membrane is discussed in the context of membrane fluidity.
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