Abstract
Biosynthesis of L-homophenylalanine by immobilized hyperthermophilic and halophilic amino acid dehydrogenase (AaDH) was studied. Polydopamine (PDA) modified MWNTs (MWNTs-PDA) was applied for AaDH immobilization. Coordinated with metal ions, MWNTs-PDA-Ni exhibit high loading capacity and biocompatibility. MWNTs-PDA-AaDH and MWNTs-PDA-Ni-AaDH enhanced activity by 3.2 and 7.4 -fold than free enzyme, respectively. Compared with the MWNTs-PDA-AaDH, nickel ions coordinated MWNTs-PDA improve enzyme recovery of immobilization by twice, and the enzyme activity remained as 58.0% after 7 recycles. Compared with free enzyme, stability of MWNTs-PDA-Ni-AaDH and MWNTs-PDA-AaDH in 30% cyclohexane were enhanced by 2.0 and 3.2 -fold, respectively. Kinetic parameters of MWNTs-PDA-AaDH, MWNTs-PDA-Ni-AaDH were caculated and compared. Mechanism of immobilization was investigated from the perspectives of multi-level interactions. This study offers an easy application of conductive hybride materials for enzyme immobilization, which can further expand the application for bioelectrodes.
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