Abstract
This chapter presents the study on characterization and isolation of liver specific proteins in different species. Antibodies were found in the sera of 16 patients out of a group of 85 cases with active chronic hepatitis. On liver biopsy, these 16 patients showed the morphological changes of chronic agressive hepatitis at different stages, and hyper-gamma globulinaemia in their serum. The antibody was an IgG type of immunoglobulin and was not absorbed by soluble proteins of other organs. Complete species specificity could not be ascertained as hetero-immune sera against human liver specific protein showed lines of identity with the antibody found in these human sera. Complete absorption of the auto-antibody is only possible with the liver specific protein. Antisera to rat liver specific protein were raised in rabbits and after absorption with rat kidney and rat plasma, those with significant titres to rat liver specific protein was used in the subsequent experiments. Immunofluorescent studies of the perfused liver showed localization of the consumed antibodies to cytoplasmic and membrane structures of the hepatocytes.
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